ICAM1 anticorps

N° du produit ABIN120499

Cet anticorps Souris Monoclonal détecte spécifiquement ICAM1 dans WB, FACS, IP, IHC (p) et IHC (fro). Il présente une réactivité envers Rat.

Aperçu rapide pour ICAM1 anticorps (ABIN120499)

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ICAM1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1A29

Détail du produit anti-ICAM1 anticorps

Specificité: This antibody recognizes the CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend SM286LE for this use.

Réactivité croisée (Details): Species reactivity (tested):Rat.

Purification: Affinity Chromatography on Protein G

Immunogène: Rat Ax cells (a HEV derived cell line). Remarks: Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 1/25 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Western Blot. Functional Assays. Immunoprecipitation. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing an thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ICAM1

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: CD54 / ICAM1

Sujet: CD54 is a 90kD adhesion molecule belonging to the immunoglobulin superfamily. CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

ID gène: 25464

NCBI Accession: NP_037099

UniProt: Q00238

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling